The Two Weeks in the World research project has resulted in a dataset of 3100 clinically relevant bacterial genomes with pertaining metadata, collected from 59 diagnostic units in 35 countries around the world during 2020. A relational database is available with metadata and summary data from select bioinformatic analysis, such as species prediction and identification of acquired resistance genes. The dataset is browsable and interactable through a web-application. This is created and shared according to the FAIR principles and has large reuse potential within the research fields of clinical microbiology and global health.