Polymer-Lipid Nanodisc Self-assembly Processes Using Stop Flow

DOI

Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Polymer stabilized lipid bilayer discs are easily made by adding polymer to a suspension of lipids or disrupted cell membranes, with discs forming within ~5 minutes. It is not clear how the polymer forms discs from these systems, since in vesicles or cell membranes the headgroups are exposed to solution, and the soluble polymer, but in the final discs, the polymer is wrapped around the tail regions of the lipid bilayer. We have also observed that nanodiscs are dynamic, and undergo exchange of lipids between discs or between discs and lipid bilayers on ~10 min timescales. Here we propose to use stop-flow on Sans2d to study nanodisc formation from a solution of vesicles mixed with polymer, and also to probe the kinetics of lipid exchange between nanodiscs.

Identifier
DOI https://doi.org/10.5286/ISIS.E.61001625
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/61001625
Provenance
Creator Professor Karen Edler; Dr Ann Terry; Ms Cecilia Tognoloni; Dr Thomas Arnold
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2015-07-07T13:56:05Z
Temporal Coverage End 2015-07-20T09:01:59Z