Changes of phospholipid aggregate morphology induced by phospholipase hydrolysis

DOI

We propose to look at the interaction of the aggregates two phospholipids form with two phospholipase (PL) enzymes, PLA2 and PLC, which hydrolyse different ester groups in the lipid molecule and therefore produce different reaction products. It is well known that the length of the fatty acid chain in a phopholipid molecule dictates the nature and size of the aggregates a phospholipid forms upon dispersal in water and consequently its interaction with PL. In the present study we have chosen two small phosphocholine lipids, one with a 7-carbon and one with a 6-carbon fatty acid chain, where the latter forms spherical micelles while the former rod-shaped micelles. SANS measurements coupled with stopped flow methodology and using contrast variation will be used to determine the kinetics of the interaction and the rate of destruction of the micellar aggregates.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24079527
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24079527
Provenance
Creator Professor Jayne Lawrence; Dr Sylvia McLain; Ms Fabrizia Foglia; Dr Chris Lorenz; Dr Fabrizia Foglia; Miss Louise Collins
Publisher ISIS Neutron and Muon Source
Publication Year 2014
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2011-10-30T11:43:36Z
Temporal Coverage End 2011-11-01T15:50:55Z