Conjugation of therapeutic proteins to polymers is well established but most methods result in multi-site and multiple conjugation, crosslinking resulting in lack of orientation and loss of activity. Using an enzyme mediated conjugation methodology we have prepared well-defined protein-polymer conjugates using a model protein, green fluorescent protein with an amphiphilic, temperature responsive diblock copolymer based on N-2-hydroxypropyl methacrylamide, a polymer commonly used for delivery of therapeutics. In this proposal we wish to determine the effects of polymer hydrophobicity on the properties of aggregates formed by these polymers & conjugates in solution, to develop an understanding of the phase behaviour of these species. Since one of the polymer blocks becomes less soluble as the temperature increases we will also study the effect of temperature on these self-assembled species