Here, we provide an improved version of the PN40024 genome assembly, called PN40024.v4, which combines the top-quality Sanger contigs from the 12X version with Pacific Biosciences long reads (Sequel SMRT). Along with this new assembly, we also provide a new version of the gene annotation, called PN40024.v4.1 based on a newly developed annotation workflow and RNA-Seq/proteins datasets. Then, the gene annotation version PN40024.v4.3 was generated. This was generated from version PN40024.v4.1, and the structure of multiple gene family catalogues was curated using the Apollo tool (manual curation of a set of genes of functional interest to the community).
bwa-mem2, v2.0
Samtools, v1.9
MUMmer, v3.1
BEDTools, v2.26.0
Trimmomatic, v0.39
GMAP/GSNAP, v2020-09-12
PsiCLASS, v1.0.1
GlimmerHMM, v3.0.4
MAKER2, v3.01.03
ncbi-blast, 2.10.1+
BRAKER2, v2.1.5-master_20200915
GeneMark-ETP +, v4.61
AUGUSTUS, master_v3.3.3_20200914
DIAMOND, v0.9.24.125
SPALN, 2.3.3f
ProtHint, 2.5.0
BamTools, v2.5.1
GeneID, v1.4.5-master-20200916
EvidenceModeler, v1.1.1
PASA, v2.4.1
tRNAscan-SE, 2.0
Blast2GO, v1.5.1
InterProScan, v5.52-86.0
BUSCO, v5.1.2
Apollo, v2.6.4
CANU, v.1.6
LORDEC, v.0.5.3
minimap2, v2.17-r954-dirty
Flye, v2.4-gc9db046
Haplomerger2, v20180603
OPERA-LG, v2.0.6
GapCloser, v1.12
LR_Gapcloser, v1.0
PILON, v1.23-1-g41e0b8e
liftoff, v1.5.1
exonerate, v2.4.0