This dataset represents the raw data analyzed for the article titled "Influence of plant identity in wheat mixtures on the control of English Grain Aphid (Sitobion avenae) ." submitted in Jorunal of Applied Entomology. The data was collected in laboratory and greenhouse studies in 2022 at the University of Agricultural Science of Sweden (SLU), Uppsala. . It contains information of the number of aphids, wheat biomass and the nitrogen and carbon content obtained in the Serveis Científico-Tècnics de la Universitat de Barcelona.