N-acyl amino acids are surfactants with attractive applications in different technological fields. They are referred to as mild surfactants because they are commonly considered less irritant and safer than sulfates anionic surfactants. N-acyl amino acid surfactants could infact represent an alternative and safer option to SDS. However, the full potential of such surfactants remains unexplored, mainly because of the lack of information regarding a full characterization of their physicochemical and toxicological properties, including their interaction with biological membranes.With the aim to use these compounds as drug delivery agents,we propose here to study their self-assembly behavior in solution. This is uniquely possible by SANS and will help understanding their shape, loading and temperature stability.This study will help us proceeding further in their application in real systems.