Rain events increase Tijuana river flow to levels that the international water treatment plant in the Mexico-US border can not process. Therefore, rain runoff and sewage polluted waters are released into the ocean untreated. Routine testing is performed on ocean water, but little is known about aerosolization of microbial and chemical entities with polluted coastal waters. Here, we assess the microbial and metabolomic characteristics of Tijuana river water and ocean water at Imperial Beach and SIO piers, as well as aerosols collected near the coastal line.