This data set comprises all fish biomass data collected during the RV ALKOR cruise AL577 conducted in July/August 2022 (28th of July to 8th of August) in the Western Baltic Sea by the University of Hamburg. The cruise was part of the 5-year MARSYS teaching cruise program of the University of Hamburg GFP20-1_047. The fish biomass data were collected directly on board. Fish biomass was taken after sorting through a complete fish haul into the single species and weighing them on a scale on board.