EMODnet Chemistry aims to provide access to marine chemistry data sets and derived data products concerning eutrophication, ocean acidification, contaminants and litter. The chosen parameters are relevant for the Marine Strategy Framework Directive (MSFD), in particular for descriptors 5, 8, 9 and 10. The datasets contain standardized, harmonized and validated data collections from seafloor litter. Datasets concerning seafloor litter data are loaded in a central database after a semi-automated validation phase. Once loaded, a data assessment is performed in order to check data consistency and potential errors are corrected thanks to a feedback loop with data originators. EMODnet seafloor litter data and database are hosted and maintained by ‘Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Division of Oceanography (OGS/NODC)’ from Italy. For seafloor litter, the harmonized datasets contain all unrestricted EMODnet Chemistry data on seafloor litter data, including 20877 CDI records. The temporal range covered is from 2006-10-01 to 2021-03-16. Data are formatted following Guidelines and forms for gathering marine litter data, which can be found at: https://dx.doi.org/10.6092/15c0d34c-a01a-4091-91ac-7c4f561ab508. The updated vocabularies of admitted values are available at https://vocab.seadatanet.org/search or at https://vocab.ices.dk/ . The harmonized datasets can be downloaded as EMODnet Sea-floor litter data format Version 1.0, which is a csv file, tab separated values. The original datasets can be searched and downloaded from EMODnet Chemistry Chemistry CDI Data and Discovery Access Service: https://emodnet-chemistry.maris.nl/search