Lipid dynamics in yeast cell membranes – effect of ergosterol and its precursors

DOI

A cell membrane can contain hundreds of different lipid molecules, but our understanding of the dynamics of cell membranes is very limited. As a result of a collaboration between the ILL and ESS on the deuteration of lipids from cell cultures, we can now produce 100s of milligrams of lipid extracts from yeast and other microbial cell cultures, sufficient for neutron spectroscopy studies of their dynamics. We recently carried out the first QENS experiment on IRIS to probe the dynamics of the phospholipid extract from P. pastoris yeast, and found that there is a significant difference in their average molecular dynamics compared to model lipids such as POPC and DMPC. We now propose to continue our investigation on IRIS to probe the effect of ergosterol and its drug-resistance related precursors on yeast phospholipid dynamics.

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1920364-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/108642967
Provenance
Creator Dr Victoria Garcia Sakai; Mr Juan Manuel Orozco Rodriguez; Dr Wolfgang Knecht; Dr Hanna Wacklin-Knecht
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2019-11-13T08:30:00Z
Temporal Coverage End 2019-11-22T09:46:32Z