Using an enzyme mediated conjugation method we have prepared well-designed protein-polymer conjugates using a model protein, green fluorescent protein (GFP) with thermo-responsive polymers based on HPMA (N-2- hydroxypropyl methacrylamide)-Acetyl-HPMA (O-acetyl-N-2-hydroxypropyl methacrylamide) and a commercially available PNIPAM-NH2.In this proposal we wish to study in greater detail the self-assembly of these temperature responsive polymer-protein conjugates. Such investigations could be particularly useful to understand the structural properties of these materials as the molecular weight changes and how they respond to the polymers transition to a collapsed state at higher temperatures. The phase diagram of eGFP-copolymer conjugates will be mapped out with respect to copolymer composition and concentration effects as well as temperature and water content.