This study aims to know the evolution of the microbiota of Yellowfin tuna steaks using the Illumina MiSeq platform to sequencing the 16S rRNA gene V1-V3 hypervariable region. Three storage conditions were analyzed: iced at 0°C (code sample = AIR) vacuum packaging (code sample = VP) modified atmosphere (70% CO2, 30% O2) (code sample = MAP). VP and MAP samples were stored at 4°C the firts week and then at 8°C, according to the French standard shelf life validation of AFNOR. Samples were analyzed before the packaging (fresh tuna) (code sample = D0), after eight days of storage (code sample = D8) and at the sensory rejection point (code sample = D13). All FastQ files are corresponding to Quality filtered reads (minimal length of 450 bp, an exact match to the barcode and 1 mismatch allowed to the proximal primer).