Dataset of the characterization of the optimum pH of reaction, optimum cofactor concentration, optimum initial enzymatic activities and ratios and the use of a sparging gas for the multi-enzymatic synthesis of acetoin from ethanol in a one-pot reactor.

METHODOLOGICAL INFORMATION

1. Description of the methods used to collect and generate the data: -Single enzymatic reaction (ZpPDC) and preliminary characterizations (ZpPDC/ScADH and ZpPDC/ScADH/SpNOX) were carried out in 50 mL using tris base buffer at different pH conditions, acetaldehyde or etanol 50 mM, at 25ºC and 500 rpm. -Multi-enzymatic system reaction was carried out in 5 mL using tris base buffer pH 7.5 and bioetanol 50 mM, with pH control, at 25ºC, 1000 rpm and 1 vvm of air. -Activities were obtained applying activity tests based on spectrophotometrical methods. -GC for the analysis of ethanol, acetaldehyde and acetoin concentration. -Protein purification was carried out in a FPLC AKTA Pure System.

2. Data processing methods: All data were processed using Excel

3. Software or instruments needed to interpret the data: Excel

4. Information about instruments, calibration and standards:

5. Varian Cary 50 Bio UV-visible spectrophotometer (Agilent®).
6. FPLC

7. GC

8. Environmental or experimental conditions: Atmospheric pressure and 25ºC.