This study aimed to quantify differences in gene expression between male and female copepods during acute heat stress.</p><p>Several egg sacs were hatched together and individual larvae isolated across 24-well plates and raised to age 25-days.</p><p>For each sex, sets of 15-20 individuals were pooled and exposed to a control (20C) or stressful temperature (33C) for 60 minutes.</p><p>Six batches of paired control-heat stress replicates were run for each sex.