Binding of DNA to Model Membranes in the Presence of Ca2+ Ions and a Membrane Potential

DOI

The aim of the present work is to gain an understanding of the interaction of DNA with the phospholipid bilayers. The effect of different forms of DNA, the presence of divalent cations, such as calcium and the presence of a membrane potential will be investigated. Neutron reflectivity experiments are proposed to determine whether the binding of DNA to zwitterionic lipids, in the presence of calcium, is affected by the presence of a bilayer and transbilayer potential. We will be able to create a transbilayer potential by varying the voltage applied to an electrode, allowing the effect of the bilayer potential on DNA binding to be assessed. The information gained through these studies will aid in our understanding of the interaction of DNA with bilayers that have physiologically-relevant potential with a view to improving the design of gene delivery vesicles.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24003087
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24003087
Provenance
Creator Dr Aleksandra Dabkowska; Dr Arwel Hughes; Professor Jayne Lawrence
Publisher ISIS Neutron and Muon Source
Publication Year 2011
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Design; Fine Arts, Music, Theatre and Media Studies; Humanities
Temporal Coverage Begin 2008-03-05T10:32:48Z
Temporal Coverage End 2008-03-07T23:55:48Z