Comparative expression analysis of HOG1 KOs during osmotic stress in the ascomycete yeasts Overall design: Gene expression was measured in KO strains as well as WT strains of three ascomycete yeasts, S. cerevisiae, C. albicans, and S. pombe. Expression was measured in a wildtype strain of each species, as well as a sko1 knockout mutant for S. cerevisiae and C. albicans, an msn2/4 knockout mutant for S. cerevisiae, an mnl1 knockout mutant for C. albicans, and an hsr1 knockout mutant for S. pombe. Expression was measured under normal media at T=0, and under KCl stress at T=20. Two replicates were used for each experiment.