Planar bilayer formation on novel cationic surface suitable for membrane protein studies

DOI

Membrane receptor proteins (MRPs) which are thought to represent the majority of drug targets are challenging to study as their structure and function is dependent on being embedded within a lipid matrix. One promising route of investigating MRPs is to embed them in planar floating bilayers. We have prepared a grafted monolayer from a novel cationic polymerisable lipid in order to aid the formation planar supported bilayers from MRPs-containing vesicles. The proposed study will test the effect of altering the charge of the grafted surfaces, as well as the charge of the vesicles themselves, on the deposition of vesicles. The overarching aim of the proposed study is to characterize the structure of the lipid bilayers deposited onto the cationic surfaces in order to determine whether they will be suitable for the deposition of vesicles containing MRPs.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24083815
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24083815
Provenance
Creator Dr Rob Barker; Professor Jayne Lawrence; Dr Dave Barlow; Dr Aleksandra Dabkowska; Dr Arwel Hughes; Miss Louise Collins
Publisher ISIS Neutron and Muon Source
Publication Year 2014
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2011-04-11T03:56:44Z
Temporal Coverage End 2011-04-15T05:11:41Z