-
mini PCR: effects of PCR rxn volume and gDNA input validation
part of mackerel paper -
The bacteria of Darwin Harbour: spatial and temporal patterns in a tropical m...
Estuaries are renowned for their complex environment and productivity. They are also the focal point of impact between the land and sea and subject to increasing human... -
2018 Australia fish microbiome study
Atl salmon study ABSTRACT Successful rearing of fish in hatcheries is critical for conservation, recreational fishing, and commercial fishing through wild stock enhancements,... -
Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribe...
combined samples sequenced with primers modified for forward barcoding on the 515f site, extended to include V5 and improved ITS -
DNA extraction comparison testing 2019-2020
Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and... -
A simple microbiome in the European common cuttlefish, Sepia officinalis
The European common cuttlefish, Sepia officinalis, is used extensively in biological and biomedical research yet its microbiome remains poorly characterized. We analyzed the... -
3D multi-omic profiling of an assemblage of Hawaiian corals
Samples were taken from species Porities lobata, Montipora capitata, Montipora flabellata and subjected to 16S sequencing for bacterial profiling and LC-MS/MS for metabolomic... -
Global patterns of 16S rRNA diversity at a depth of millions of sequences per...
The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In... -
Knight skin biogeography comparison
Knight_skin_biogeography - Comparison of skin samples from a rat (mammal), cyclostomid (fish), iguana (reptile), bull frog (amphibian) and a bird (pigeon) to determine if the... -
Earth Microbiome Project Multi-omics (EMP500)
The Earth Microbiome Project Multi-omics component (EMP500) involves amplicon and shotgun metagenomic sequencing and metabolomic profiling of over five hundred microbial... -
Microbial effects of livestock manure fertilization on freshwater aquaculture...
The majority of seafood is produced from farming, with most finfish coming from freshwater ponds. Across continents, aquaculture is growing fastest in Africa at 11.7 % annual... -
Bioaerosol in Tokyoskytree
Tokyo is located in southeastern of the Japanese main island and capital of Japan (Population: 13.61 million). The climate of Tokyo is warm and humid in summer and cool and dry... -
Evaluation of the safety and efficacy of fecal microbiota transplantations in...
Gastrointestinal disease is a leading cause of morbidity in bottlenose dolphins (Tursiops truncatus) under managed care. Fecal microbiota transplantation (FMT) holds promise as... -
Song Colorado freshwater fish
Microbiota of slime and gut samples from different species of freshwater fish from the Colorado River. -
Fish microbiota 101: host traits, ecology, and environmental factors influenc...
Fish are the most diverse and widely distributed vertebrates, yet little is known about the microbial ecology of fishes nor the biological and environmental factors that... -
Knight_extraction_test
One plate of samples was extracted using several different extraction tools (new, faster) for comparison with the existing pipeline -
Microbial diversity in arctic freshwaters is structured by inoculation of mic...
A 12-year sample archive of DNA from Alaskan arctic tundra ecosystems are available to the Earth Microbiome Project for comparison of microbial diversity across a range of... -
Tijuana River pollution at Imperial Beach 2019
Rain events increase Tijuana river flow to levels that the international water treatment plant in the Mexico-US border can not process. Therefore, rain runoff and sewage... -
Patterns of microbiome transition along salinity gradients, and importance fo...
We are interested in whether the host fish microbiome is important for host adaptation to alternate osmotic niches. Toward this goal, we seek to describe the patterns of... -
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,...