We examined whether warming, acidification, and different food availability regimes interacted to affect the survival, development, and growth of larval crown-of-thorns starfish, Acanthaster sp. (CoTS). Larvae were reared in all combinations of two temperatures (26, 30 °C), two pH levels (pH 8.0, 7.6), and three food regimes ('low' ration: 1,000 cells mL-1; 'switch' ration: 1,000 cells mL-1 until day 11, followed by 50,000 cells mL-1; and 'high' ration: 50 000 cells mL-1).
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-09-06.