Effect of glycolipids with therapeutic potential on phase behaviour of model membranes

DOI

alpha-galactosylceramide (a-GalCer) can activate an immune response through a protein-mediated cell signalling event. The effect of a-GalCer on local membrane structure is poorly understood but has implications for the co-localisation of the lipid and protein. Our research aims are to elucidate the effect that a-GalCer has upon the phase behaviour of model membranes and find differences between a-GalCer and naturally occurring analogues that do not elicit the same immune response. The aim of this experiment is to determine the organisation of molecules within monolayers composed of two synthetic glycolipids and their mixtures with DPPC, using neutron reflectivity. These results will be combined with our x-ray reflectivity data to yield structural information that will enable us to interpret macroscopic behaviour and so build a more complete model of a-GalCer behaviour in membranes.

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1910356-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/103212212
Provenance
Creator Dr Maxmilian Skoda; Dr Liam Cox; Dr Thomas Arnold; Dr Mary Wood; Mr Thomas Howitt; Dr Sarah Horswell; Miss Alexandra Martin
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2019-06-07T08:00:00Z
Temporal Coverage End 2019-06-10T07:19:28Z