Mechanism and rates of phospholipid exchange and flip-flop under physiological conditions

DOI

Variation in lipid composition between different biological membrane and across membranes are vital for a wide range of cellular functions. Significant differences in lipid distribution have been found between different organelles and cells, yet the mechanism and rate of phospholipid flip-flop (movement of a lipid from one leaflet to the other) and exchange (movement of a lipid from one membrane to another) are currently very difficult to measure, and estimated rates span several orders of magnitude. Labelling lipids (e.g. with fluorescent groups) significantly affects the kinetics of these processes and so it is extremely important to develop chemical-label free methods to elucidate their mechanisms and kinetics. We aim to apply a neutron contrast variation based to probe the mechanism of inter-vesicle lipid exchange and intra-vesicle flip-flop, and systematically measure their rates.

Identifier
DOI https://doi.org/10.5286/ISIS.E.90592742
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/90592742
Provenance
Creator Mr Mohit Devgan; Miss Jenny Liu; Miss Zexi Xu; Dr Robert Law; Professor John Seddon; Dr Sarah Rogers; Dr Oscar Ces; Dr Nick Brooks; Dr Arwen Tyler; Mr Alessandro Gulotta; Professor Robert Law
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2018-03-13T09:00:00Z
Temporal Coverage End 2018-03-16T09:11:37Z