Summary: Titanium implants are surgically important and require a hydrophilic polymer coating to prevent biofouling in situ. Bioadhesive proteins can be covalently tethered to the polymer to aid implant-tissue integration. It is not known if protein conjugation changes the polymer layer, or what orientation the proteins exist in for cell adhesion. This proposal will characterise these polymer/protein coatings on titanium surfaces. Objectives: 1) Characterise a key bioadhesive protein (FIII9¿10) at the bare titanium surface (control), over various concentrations, with 2 subphase contrasts, determining protein fraction, orientation and surface excess. 2) Characterise the polymeric layer synthesised on the titanium surface in situ before and after covalent conjugation of FIII9¿10, determining polymer layer thickness and density, and protein parameters as in (1).