Raw data from a series of experiments aimed to evaluate the feasibility of producing MELs in solid-state fermentation using M. bullatus and U. maydis.

METHODOLOGICAL INFORMATION

Experimental work involved solid-state fermentation (SSF) using Moeszyomices bullatus yeast and Ustilago maydis fungi. Winterization oil cake was autoclaved and subsequently employed as oil source for the time course set up in each microorganism. For U. maydis, 2 glucose conditions were tested. MELs-crude resulted were quantificated by solvent extractions and lately analysed in UHPLC-ESI-MS for MELs congeners identification.

1. Description of methods used for collection-generation of data: Lab scale experiments were carried out using 5 0.5-L reactors.

2. Methods for processing the data:

3. MELs-crude and remaining oil were analytically determined after solvents extraction using ethyl acetate and n-hexane.
4. MELs concentration concentration was quantified by HPLC-UV.
5. Glucose was quantified by YSI equipment.

6. MELs congeners identified by UHPLC-ESI-MS

7. Instrument- or software- specific information needed to interpret the data:

8. Excel or similar soft.

9. Instruments, calibration and standards information:

10. Analytical balance Sartorius Entris II
11. Orbital shaker (Infors HT, UK)
12. HPLC UltiMateTM 3000
13. Macherey-Nagel™ Nucleosil™ 100 mm × 3 μm x 4.7 mm C18 EC column

14. FTIR Spectrophotometer (Tensor 27, Bruker) with MKII Golden Gate

15. Environmental or experimental conditions:

16. M. bullatus CECT 13162 was cryopreserved at -80°C.
17. U. maydis CECT 20750 was cryopreserved at -80°C.
18. Microorganism growing conditions were set up at 30 ºC, 180 rpm at 48 h.

19. 0.5-L bioreactors operational parameters were established at: temperature 30 ºC, humidefied airflow rate 30 mL/min.

20. Quality-assurance procedures performed on the data: All time course experiments were carried out using 5 reactors, sacrificing each one per day.