Raw data from a series of experiments aimed for isolation of thermophilic biosurfactant producing microorganisms. Compost samples collected from food waste composting facility in a primary school in Barcelona and textile industry sample from Faisalabad Pakistan were used for isolation of 43 isolates were screened through biosurfactant qualitative screening, but 18 isolates dominated with promising results which were further shortlisted under solid-state culture to select the five significant performers belonging to Bacillus sp., Aspergillus sp., and Streptomyces sp.
METHODOLOGICAL INFORMATION
Experimental work involved sample collection from community composter treating food waste, Fiber-like material from a textile industry, and samples from enrichment experiments for isolation of biosurfactant producing microorganisms. The experimental setup involved submerged fermenatation in 250 mL erlenmeyer flasks with 50 mL minimal salt medium and glucose as carbon source. Followed by screening of biosurfactant producers a time course solid state fermentation experiment was performed using wheat bran, wheat straw, and WOC as substrate supplimented with minimal salt medium in 0.5 L bioreactors.
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Description of methods used for collection-generation of data:
Lab scale experiments were carried out using 250 mL Erlenmeyer flasks with a total working volume of 50 mL media. Then, a time course fementation was performed at 0.5-L Batch bioreactor.
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Methods for processing the data:
- Cell dry weight results were analyzed by gravimetrical quantification.
- Biosurfactant concentration were analytically determined after solvents extraction using ethyl acetate and n-hexane.
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Biosurfactant and glucose concentration was quantified by HPLC-UV and qualitative assays.
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Instrument- or software- specific information needed to interpret the data:
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Excel or similar soft.
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Instruments, calibration and standards information:
- Analytical balance Sartorius Entris II
- Orbital shaker (Infors HT, UK)
- Centrifuge (Sigma 6–16S, SciQuip, UK).
- HPLC UltiMateTM 3000
- Macherey-Nagel™ Nucleosil™ 100 mm × 3 μm x 4.7 mm C18 EC column
- Aminex HPX-87 P column (Biorad, USA) coupled to a refractive index detector (RI)
- Multi-N/C 2100S analyzer (Analytik Jena, INYCOM, Instrumentación y Componentes, S.A, Spain)
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0.5-L bioreactor (BioStat® B, Sartorius, Germany)
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Environmental or experimental conditions:
- microorganisms isolated and characterized were cryopreserved at -80°C.
- Microorganism growing conditions were set up at 50 ºC, 180 rpm at 24 h.
- Hydrolysis conditions were 55°C, 24 h and 180 rpm.
- Fermentations in 250 mL Erlenmeyer flasks were conducted at 50 ºC, 168 h, and 180 rpm.
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0.5-L bioreactor operational parameters were established at: temperature 50 ºC, airflow rate 0.3-L air/kg , dissolved oxygen level was consistently maintained above 20%.
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Quality-assurance procedures performed on the data:
All experiments were carried out in duplicate to estimate pure error.