Human telomeric G-quadruplex complexed with the highly selective anticancer drug BRACO-19. A SANS investigation along the melting path.

DOI

G-quadruplexes (G4) are the focus of a number of studies in both fundamental and applied research, from cancer biology and novel therapeutics through to nanotechnology. The G4 topology depends on a variety of factors, such as the temperature and the specific interaction with ligands, i.e. potential anti-cancer drugs. Quite recently we have shown that in the case of the human telomeric quadruplex Tel22 both the free system and the one complexed with a model drug undergo melting through a multi-state process. This information is crucial to correctly modelize the Tel22 stability. Here we ask to do a SANS investigation of the complexation of Tel22 with BRACO-19, a potent stabilizer ligand with a remarkable in vivo single-agent antitumor activity. SANS measurements along the thermal unfolding path will reveal the large-scale structural features of the native and intermediate states.

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1920368-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/108678311
Provenance
Creator Dr Lucia Comez; Dr Najet Mahmoudi; Professor Francesco Spinozzi; Professor alessandro paciaroni
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2019-11-16T09:00:00Z
Temporal Coverage End 2019-11-18T10:37:39Z