The dataset contains multimodal and multidimensional microscopy images of the interactions between different microparticles either in skin keratocytes cells (SKC) or in corneal keratocyte cells (CKC) of Atlantic Salmon. All images were captured at room temperature, and the dataset includes both test (with microparticles) and control (without microparticles) conditions. These interactions were captured using two different microscopes: 1) 3D Cell Explorer-fluo (Nanolive): a holotomographic imaging method which produces label-free 3D images using refractive index as the contrast mechanism and can optionally also provide 2D correlative fluorescence images. 2) DeltaVision Elite (GE Healthcare): a widefield fluorescence microscope with integrated deconvolution for improved 3D imaging, which can provide correlative 2D differential interference contrast (DIC) images. The main goal of this study was to understand how different microparticles interact with SKC and CKC, which are two of the important defense cells for innate immunity in fish. This dataset may be useful for researchers interested in studying salmon skin and corneal cell behavior, those who are interested in cell-microparticle interactions, as well as those interested in accessing or processing a relatively new type of microscopy image (holotomography).

Fiji/limageJ, https://imagej.net/ij/features.html

steve, https://www.nanolive.ch/products/3d-microscopes/cx/