Direct Observation of Gram Negative Bacterial Membrane Disruption by Supramolecular Antimicrobials

DOI

Cyclic peptides with alternating side chain stereochemistry are capable of self-assembling into long nanotubes. The conjugation of polymers to these peptides renders the resulting nanotubes water soluble, whilst providing functionality. In response to the rising global threat of increasing antimicrobial resistance, we have been developing cationic cyclic peptide-polymer conjugates to target anionic Gram-negative bacterial membranes as supramolecular antimicrobials. Here, we propose to assemble asymmetric planar floating lipid bilayers on functionalised gold surfaces to mimic the outer membrane of Gram-negative bacteria. By using neutron reflectometry and exploiting isotopic contrast, we aim to determine the mechanism of membrane disruption by these materials whilst obtaining information relating the molecular properties of cyclic peptide-polymer conjugates to their antimicrobial activity

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1920533-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/108679365
Provenance
Creator Mr Thomas Floyd; Professor Sebastien Perrier; Dr Alexia Hapeshi; Mr Ramon Garcia; Dr Stephen Hall; Dr Luke Clifton
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2019-12-07T08:30:00Z
Temporal Coverage End 2019-12-13T12:08:48Z