Lysylphosphatidylglycerol (LPG) is a major constituent of the plasma membrane of the bacterial pathogen methicilin resistant Staphylococcus aureus (MRSA), which has been implicated in their resistance to a number of cationic drugs including antimicrobial peptides. Studying the effect of LPG on membrane behaviour and determining its precise role in MRSA drug resistance mechanisms, has hitherto been hampered by the labile nature of the lipid, which is readily hydrolysed under mild aqueous conditions. We have succeeded in synthesising a stable analogue of LPG, called LPPPA, which is suitable for use in biophysical experiments aimed at elucidating the behaviour and functions of the native lipid.