Electrostatic Interactions between Floating Bilayers and Thiol Coated Interfaces Controls water Gap Thickness

DOI

Bilayers which float below a self-assembled monolayer (SAM) coated solid support represent one of the most advanced membrane mimetics. Recently (RB 1720155) we assessed the feasibility of depositing floating supported membranes below self-assembled monolayers of (15-Mercaptopentadecyl)-1-aminoethanol (ThiolC15AmideAlcohol). We plan to continue this research by creating eight differing model bilayer systems at the gold/water interface and examining the structures of these biomembrane mimetics using. Each bilayer will be deposited below both pure ThiolC15AmideEthanol SAMs and a mixed 50:50 mixed ThiolC15AmideEthanol and ThiolC15AmideAmine SAMs. We wish to access the relationship between bilayer-SAM electrostatics and water gap distance. From this we aim to determine which sample and SAM conditions are optimal for producing stable floating membranes for a diverse range of lipid species.

Identifier
DOI https://doi.org/10.5286/ISIS.E.98001709
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/98001709
Provenance
Creator Dr Jos Cooper; Dr Martynas Gavutis; Dr Luke Clifton; Dr Nico Paracini; Dr Nina-Juliane Steinke; Professor Jeremy Lakey
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-10-18T07:00:00Z
Temporal Coverage End 2018-10-22T07:39:07Z