Phaeocystis pouchetii's responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm)

We assessed the responses of solitary cells of Arctic Phaeocystis pouchetii (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry.

Identifier
DOI https://doi.org/10.1594/PANGAEA.948489
PID https://hdl.handle.net/10013/epic.27912.d001
Related Identifier IsSupplementTo https://doi.org/10.1002/lno.12460
Related Identifier References https://doi.org/10.1139/m62-029
Related Identifier References https://doi.org/10.1111/nph.13885
Related Identifier References https://doi.org/10.4319/lom.2012.10.142
Related Identifier References https://doi.org/10.3334/CDIAC/otg.CO2SYS_XLS_CDIAC105a
Related Identifier References https://doi.org/10.4319/lo.2012.57.2.0607
Related Identifier References https://doi.org/10.1021/ac010303r
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.948489
Provenance
Creator Rokitta, Sebastian D ORCID logo; Großmann, Christian H; Werner, Elisa; Moye, Jannika; Castellani, Giulia (ORCID: 0000-0001-6151-015X); Nöthig, Eva-Maria ORCID logo; Rost, Björn ORCID logo
Publisher PANGAEA
Publication Year 2022
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 908 data points
Discipline Chemistry; Design; Fine Arts, Music, Theatre and Media Studies; Humanities; Natural Sciences
Spatial Coverage (5.994 LON, 78.835 LAT); Fram Strait
Temporal Coverage Begin 2016-05-10T00:00:00Z
Temporal Coverage End 2017-09-15T00:00:00Z