Soft self-assembled structures have been extensively used as drug nanocarriers. Their building blocks, naturally occurring and synthetic amphiphiles (lipids or block copolymers), have been proven to offer a wide range of morphologies on the nanometre scale. Careful design of the amphiphiles’ molecular structure and composition allows for self-assembly or structural transition of such nanoconstructs upon external stimuli. Here, we propose a study to characterize the effect of composition and temperature on the self-assembly behaviour of phospholipids and temperature-responsive triblock copolymers. Besides answering fundamental questions on the structure morphology of such nanoconstructs, the results of this study will be particularly important for the design of novel drug nanocarriers with tunable morphology and versatile applicability over a wide range of projects within our group.