Molecular Mechanistic Processes of Complexation between DNA and Cationic Copolymers

DOI

We have previously used LOQ to identify the complex (polyplex) structures formed between a short single strand DNA and a cationic diblock copolymer MPC30-DEA60. The SANS measurements allowed us to determine the size and shape of the aggregates formed under different charge ratios. We propose to extend this study to the assessment of the effect of the size of DEA using the copolymer series MPC30-DEA30, MPC30-DEA60 and MPC30-DEA100. Deuterated copolymer samples will be used to reveal the more detailed structures of the polyplexes formed. This study will lead to a better understanding of the contributing factors (size and concentration of polyplexes and their charges) on cell transfection efficiency.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24070506
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24070506
Provenance
Creator Professor Jian Lu; Dr Xiubo (Jon) Zhao; Ms Amy Freund
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-07-17T11:19:03Z
Temporal Coverage End 2009-07-27T08:05:35Z