Illumina and PacBio sequences for haddock

200 bp insert size paired end libraries were constructed with Illumina DNA paired end sample preparation reagents and sequenced at the McGill University and Génome Québec Innovation Centre, both 100 bp and 150 bp reads. The 3 kbp and 10 kbp insert size libraries were prepared with the Illumina Mate Pair gDNA reagents and sequenced at the McGill University and Génome Québec Innovation Centre with 100 bp reads. All Illumina libraries were sequenced on the HiSeq 2000 using V3 chemistry. PacBio SMRT sequencing was performed on a PacBio RS II instrument (Pacific Biosciences of California Inc., Menlo Park, CA, USA) at the Norwegian Sequencing Centre (NSC, www.sequencing.uio.no/). Long insert SMRTbell template libraries were prepared at NSC according to PacBio protocols. In total, 24 SMRT-cells were sequenced using P6v2 polymerase binding and C4 sequencing kits with 120 min acquisition. Approximately 16.4 Gbp of library bases were produced.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012DF0CC38460A55C2E816D30305178C3232B93AE5D
Metadata Access https://data.blue-cloud.org/api/collections/DF0CC38460A55C2E816D30305178C3232B93AE5D
Provenance
Instrument PacBio RS; Illumina HiSeq 2000; ILLUMINA; PACBIO_SMRT
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Coverage Begin 2017-07-07T00:00:00Z
Temporal Coverage End 2017-07-13T00:00:00Z