The interfacial self-assembly between large (polymer) and small (peptide) molecules of opposite charge results in the formation of highly organized membranes. Furhter manipulation of the peptide sequence leads to membranes with distinct surface morphologies, from well-defined micro-grooves to micro-sized aggregates.This proposal aims to investigate how peptide sequence controls the behavior and organization of peptide amphiphiles at the air-water interface and understand their interactions with aqueous polymer solutions.The ability of modulating the surface topography of self-assembled membranes, by introducing subtle changes in the peptide sequence, offers a simple way to develop patterned thin soft membranes with great potential for biomedical applications.