Interfacial properties of a lung protein after exposure to gas-phase ozone

DOI

Lung surfactant protein B, SP-B, is found at the air-water interface of the lung. A deficiency in SP-B at birth is known to be fatal. We have recently successfully studied the ozone-initiated oxidation of the N-terminal portion of SP-B, SP-B1¿25, at the air-water interface and shown that parts of the peptide reacts rapidly with ozone and the reaction leads to a change in surface structure. Surface pressure measurements indicate that the oxidized peptide has different surface properties to the native forms. In this work we propose to study the surface properties of monolayers of lung lipid DPPC and SP-B(1-25) after exposure to gas phase ozone. We wish to ascertain whether the oxidized peptide is more readily squeezed out of the monolayer than SP-B(1-25) itself and how readily the oxidized peptide is re-adsorbed to the interface when the surface pressure is decreased.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24088581
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24088581
Provenance
Creator Dr Arwel Hughes; Dr Katherine Thompson; Professor Adrian Rennie; Miss Joanna Hemming
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2012-03-29T13:00:30Z
Temporal Coverage End 2012-05-18T06:54:28Z