With more than 30,000 species, fish are the largest and most ancient vertebrate group. Here, we announce the 10,000 Fish Genomes Project (Fish10K), aiming to sequence 10,000 representative fish genomes in a systematic fashion and the goal of the Fish10K is to high-quality reference genomes. Through developing and applying effective analysis methods, we will be able to address critical evolutionary and biological research questions related to fish. The project officially welcome collaborators to join this effort. This BioProject ID will provide detailed information such sequencing data and assemblies, as well as continuously updated information on the sequenced species and will be publicly available to the community.