Assembling floating, very sparsely tethered, bilayers.

DOI

The cell membrane is a very important structure in biology and many laboratory based model systems have been developed to study it using biophysical techniques. Our particular target is the bacterial outer membrane which is a significant barrier to antibiotics. Recently we have succeeded in making a realistic model but, to improve it further, we need to enlarge the water filled compartment below the model outer membrane which imitates the bacterial periplasm. Methods exist to anchor membranes above water layers but so far the density of anchoring molecule has been too high (>40%) to properly form a realistic periplasm. Here we wish to test a new very sparse anchoring method which works with a surface containing only 1% anchoring molecules. If this works we hope to extend the length of the spacers to control the size of our model periplasm.

Identifier
DOI https://doi.org/10.5286/ISIS.E.73946018
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/73946018
Provenance
Creator Dr Martynas Gavutis; Dr Luke Clifton; Professor Jeremy Lakey; Dr Nico Paracini
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2016-03-03T08:00:00Z
Temporal Coverage End 2016-03-05T08:00:00Z