Bacterial and archaeal 16S rRNA from Arctic Ocean water column and subsurface sediment

Samples were collected at eight locations along Langseth Ridge, Arctic Ocean using a CTD and Multicorer (MUC). Water samples were extracted using the PowerWater® DNA Isolation Kit (Qiagen, Germany) and the MUC samples were extracted using the MoBio FastDNA™ Spin kit (MP Biomedicals, USA). Thereafter, DNA was subjected to Illumina Miseq library preparation and paired-end tag sequencing (2 x 300 bp), in triplicate, at the Centrum für Biotechnologie (CeBiTec University of Bielefeld, Bielefeld, Germany). We used multiple primers to target different regions of the 16S rRNA gene.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0123F05BCE1715DF4A301B0B0810EB77E2059825F34
Metadata Access https://data.blue-cloud.org/api/collections/3F05BCE1715DF4A301B0B0810EB77E2059825F34
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (55.779W, 85.206S, 62.014E, 87.105N)
Temporal Coverage Begin 2016-09-15T00:00:00Z
Temporal Coverage End 2019-10-21T00:00:00Z