The elasmobranchs are typically found within marine environments. However, some species, such as the Spiny dogfish (Squalus suckleyi) have been found in waters that are brackish. The underlying mechanisms that these animals use to deal with the alterations in osmotic gradients is not clearly understood. This study used RNA-seq to compare the transcriptomic changes from baseline (0 hours in 100% salt water) to short (12 hours) and long (48 hours) term exposure to 65% salt water in kidney tissue.