Structural characterisation of a transmembrane biological electron conduit using SANS

DOI

Many bacteria are able to respire using solid minerals and metals as electron acceptors in the absence of oxygen or other diffusible substrates. In Shewanella oneidensis this respiration requires a porin-cytochrome complex that assembles in the outer membrane. This complex is composed of a trans-membrane porin MtrB, a periplasmic deca-haem cytochrome MtrA and a surface exposed outer membrane cytochrome MtrC. We intend to obtain information on the shape and size of the MtrCAB complex, which will inform on the orientation of exposed MtrC as well as whether MtrA either fully or partially enters MtrB, thus providing evidence for MtrB functioning as a sheath for MtrA. We intend to perform small angle neutron scattering experiments using the SANS2D instrument to generate scattering profiles and ultimately a molecular surface model for this membrane protein complex.

Identifier
DOI https://doi.org/10.5286/ISIS.E.49914001
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/49914001
Provenance
Creator Dr Stephen Carr; Dr Tom Clarke; Dr David Scott; Dr Marcus Edwards; Mr Matthew Lawes; Dr Gaye White; Dr DENIS PTCHELKINE; Miss Nishtha Gulati; Dr Sarah Rogers; Dr Richard Heenan
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2015-04-15T08:00:00Z
Temporal Coverage End 2015-04-16T08:00:00Z