In the present study, four important somatotropic and reproductive tissues including brain, liver, gonad and muscle from 3 females and 3 males were used for transcriptomic analysis. A mean of 47,102,115 high quality clean reads were obtained from each library and 156, 67, 3434, and 378 differentially expressed genes in female and male were identified from brain, liver, gonad, and muscle, respectively (q < 0.05). These genes were further enriched in to ion channel activity, protein binding, lipid transporter activity, and glycolytic process GO terms. The KEGG enrichment analysis revealed that insulin secretion, Calcium signaling pathway, Signaling pathways regulating pluripotency of stem cells, Glycolysis / Gluconeogenesis were significantly enriched. Overall design: Examination of four important somatotropic and reporductive tissues including brain, pituitary, liver and gonad from 3 females and 3 males Starry Flounder