We studied the distribution of coccoliths in surface sediments across the Drake Passage and calcification of Emiliania huxleyi morphotypes in surface sediment samples retrieved during PS97.Dataset 1 (morphometrical measurements and mass estimations) is reporting the morphometrical measurements of E. huxleyi coccoliths measured with the Coccobiom2 macro (Coccobiom2 macros: http://ina.tmsoc.org/nannos/coccobiom/Usernotes.html, last access: 3 September 2016) based on 570 Scanning Electron Microscope images (showing E. huxleyi coccoliths of morphotypes A, Aovercalcified, B/C and O), and calcite mass estimations based on two different formulas 1) after Beuvier et al. 2019 (doi:10.1038/s41467-019-08635-x) and 2) after Young and Ziveri 2000 (doi:10.1016/S0967-0645(00)00003-5). The morphometrical measurements (in µm) include coccolith distal shield length, distal shield width, Central Area length, Central Area width. The mass estimations (in pg) include the mass calculated after 1) and 2) and the respective shape factor used for 2). The coccoliths stem from surface sediments that were sampled with a Multicorer and are approximately of Mid to Late Holocene Age.Dataset 2 (morphotype counts) is reporting the relative number of E. huxleyi morphotypes per sample, based on an additional count with the SEM. Further details in the material and methods section in the corresponding paper.