A series of well defined comb polymers consisting of methacrylate backbones and oligo(2-ethyl-2-oxazoline) side chains was shown to exhibit lower critical solution temperature behavior in water. A cylindrical conformation of the polymers in aqueous solution depending on the chain length was already confirmed by SANS using the SANS Xpress service. DLS measurements at varying temperatures below the cloud point (Tcp) of the solutions revealed the presence of single chains and larger agglomerates hinting towards a possible pre-organization of the comb polymers even below Tcp. SANS measurements at different temperatures below Tcp are proposed to elucidate those changes of the molecular structure from cylindrical bottle-brushes at lower temperatures to collapsed globules at higher temperatures. In addition, the effect of polymer chain length on its conformation will be studied in detail.