Study of the SKUD affecting the sea cucumber Holothuria scabra in Madagascar (metagenomic shotgun analyses)

Four H. scabra integument samples were analysed:</p><p>(i) ulcerated integument, individual 1/replicate 1 (1RU)</p><p>(ii) ulcerated integument, individual 2/replicate 2 (2RU)</p><p>(iii) healthy integument, individual 1/replicate 1 (1RS)</p><p>(iv) healthy integument, individual 2/replicate 2 (2RS)</p><p>Shotgun metagenome libraries were prepared and sequenced on Illumina NovaSeq 6000 platform (2x150 bp) at Eurofins Genomics (www.eurofinsgenomics.eu). Raw data were uploaded as FASTQ after demultiplexing of paired-end reads. Quality processing (i.e. dynamic trimming: minimum quality = 15, maximum low-quality basepairs = 5 detection and removal of adapter sequences using Skewer with default parameters 26) and deduplication was performed using MG-RAST 4.0.3 pipeline analysis.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0124F31370123CFF53A6EFFB8E2D3A2AC6B260860FB
Metadata Access https://data.blue-cloud.org/api/collections/4F31370123CFF53A6EFFB8E2D3A2AC6B260860FB
Provenance
Instrument Illumina NovaSeq 6000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2017-07-15T00:00:00Z