Every year, the textile, tannery and paper sectors generate massive amounts of coloured effluent, which can disturb natural ecosystems. The goal of this work is to isolate and characterize at molecular level a microbial consortium isolated from the Red Sea, Jeddah, KSA, that can remove mixed textile dyes (malachite green, cotton blue, lanazol red, Indigo carmine and bromocresol green) in artificial seawater. The optimization using Box-Behnken design and RSM was applied to identify the essential factors influencing dyes removal and to assess the interaction of these parameters and their combined effect on colour removal.