The dataset comprises data on the substrate type, temperature, salinity and benthos from 77 samples colected in 3 fixed points (P1 - 44.2357 Lat. N, 28.6288 Long. E, depth - 1.5 m; P2 - 44.2388 Lat. N, 28.6363 Long. E, depth - 4.5 m; P3 - 44.2416 Lat. N, 28.6447 Long. E, depth - 8 m) in the period 20.10 1959 - 2.11.1961, in Mamaia sector (Constanta, Romanian Black Sea Coast). The sediment samples were taken with a Van-Veen grab, the sampling size of the first 28 samples was - 0.1 m2, while for the rest of 29 - 77 samples it was 0.05 m2.. The samples have been preserved with 10% buffered formalin, then sieved through a 1 and 0.25 mm mesh sieve and subsequently stored in 70% ethanol. The database comprises taxonomic and quantitative data (individuals count, density and wet weight biomass per square meter) for 30 macrobenthic species and 6 supraspecific groups.