(1) Are there differences between sediment and water environmental DNA (eDNA) samples in terms of (a) number of reads assigned to metazoans, non-metazoans, and unclassified, (b) the number of metazoan species detected, (c) metazoan community composition? (2) Does eDNA-based species richness decrease with sediment depth, similar to trends observed in morphology-based studies ? (3) Do environmental parameters (e.g. depth, distance to open ocean) influence community composition in water eDNA?