Understanding an-harmonic behaviour in lyophilised proteins - a high energy resolution study

DOI

By combining OSIRIS/IN16 elastic window and QENS data we better understand dynamics in lyophilised apoferritin. This complimentary approach reveals that the observed decrease in elastic scattering intensity, I(Q, 100<T<300K), is due to CH3 dynamics. To compliment this work we wish to study the interplay of structure and protein dynamics. We have used OSIRIS to probe an-harmonic behaviour in superoxide dismutase (SOD, helical secondary structure) and green fluorescent protein (GFP, non-helical beta-sheet). Unlike apoferritin, both proteins show a decrease in I(Q,100<T<300K) which cannot be described simply using a CH3-activation model. This result suggests the presence of additional dynamic contributions. The OSIRIS energy resolution is insufficient to separate additional dynamic components. We therefore seek access to higher energy resolution spectroscopy (IRIS) to elucidate a difference

Identifier
DOI https://doi.org/10.5286/ISIS.E.24079352
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24079352
Provenance
Creator Professor Sue Kilcoyne; Dr Mark Telling; Dr Cameron Neylon; Dr Luke Clifton; Mr Kevin Ellis
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2010-08-04T08:54:06Z
Temporal Coverage End 2012-11-26T15:55:09Z