Doxycycline is a wide-spectrum, widely used antibiotic. In its hydrochloride form it can be used as a model for characterizing antibiotic-protein interactions. We have collected high quality, high resolution X-ray data for the compound, a prerequisite for obtaining aspherical model of experimental electron density distribution and very accurate description of the electrostatic interactions in the crystal lattice. The high quality neutron data are crutial for such study, providing information on hydrogen atom positions and atomic displacements that cannot be gained by alternative approaches. We propose to conduct a series of single crystal neutron diffraction experiments at various temperatures in order to obtain accurate hydrogen atom positions and gain insight into the proton dynamics in the complex system of hydrogen bonds in the crystal structure of the doxycycline hydrochloride.